Quantitation of the endopeptidase activity generating γ-endorphin from β-endorphin in rat brain synaptic membranes by a radiometric assay
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چکیده
منابع مشابه
β-endorphin neuron transplantation
We summarize here our new discovery that the endogenous opioid peptide β-endorphin (BEP), by virtue of reducing body stress and maintaining active immune system, as well as, balancing the levels of pro-inflammatory and anti-inflammatory cytokines, destroys tumor cells and stops them from being transformed into metastatic cancer cells.
متن کاملTransport of CRH from mouse brain directly affects peripheral production of β-endorphin by the spleen.
The blood-brain barrier (BBB) regulates the passage of substances between the brain and the periphery. It has not been shown that the secretion from the brain of a small amount of a substance can directly affect the periphery by transport across the BBB. We found that central injection of radioactively labeled corticotropin-releasing hormone (CRH) resulted in the accumulation of intact CRH in t...
متن کاملIdentification of endorphin acetyltransferase in rat brain and pituitary gland.
An enzyme which N-acetylates beta-endorphin has been identified and characterized in the rat brain and pituitary gland. The beta-endorphin acetyltransferase activity was localized almost exclusively in the intermediate lobe of the pituitary gland. beta-endorphin acetyltransferase activity was also present in the hypothalamus, a brain region which synthesizes beta-endorphin, but not the cerebell...
متن کاملModulation of Brain β-Endorphin Concentration by the Specific Part of the Y Chromosome in Mice
BACKGROUND Several studies in animal models suggest a possible effect of the specific part of the Y-chromosome (Y(NPAR)) on brain opioid, and more specifically on brain β-endorphin (BE). In humans, male prevalence is found in autistic disorder in which observation of abnormal peripheral or central BE levels are also reported. This suggests gender differences in BE associated with genetic factor...
متن کاملBIOSYNTHESIS AND RELEASE OF P-ENDORPHIN-, N-ACETYL p- ENDORPHIN-, /3-ENDORPHIN-(l-27)-, AND N-ACETYL P-ENDORPHIN- (l-27)-LIKE PEPTIDES BY RAT PITUITARY NEUROINTERMEDIATE LOBE: P-ENDORPHIN IS NOT FURTHER PROCESSED BY ANTERIOR LOBE’
Continuous labeling and pulse-chase techniques were employed to study the synthesis and secretion of multiple forms of immunoreactive ,f?-endorphin by cultured dispersed rat anterior lobe cells and intact neurointermediate pituitary lobe. Cell and medium extract immunoreactive pendorphin (specific immunoprecipitation and radioimmunoassay) exhibiting a K,, similar to authentic ,&endorphin upon g...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1984
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90417-2